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Department of Pharmacology

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Department Overview

The heart, an essential organ in humans, beats about once per second, continuously supplying oxygen and nutrients throughout the body. Various ion-regulating proteins such as ion channels, ion transporters, and Ca2+ sensor proteins that regulate these functions are present. These proteins are highly expressed not only in the heart but also in the brain and nervous system, contributing to the regulation of excitability and higher functions such as memory and learning. Abnormalities in these proteins are known to cause various cardiac and neurological disorders, including arrhythmias, heart failure, and memory impairments (Figure 1). Despite known correlations with physiological functions and pathological states, many proteins remain poorly understood, especially those that are upregulated only during disease or in immature stages, leaving intriguing questions unanswered. 

Since 2020, under the leadership of Professor Tomoe Nishitani (working name: Tomoe Y. Nakamura), the Department of Pharmacology has been researching novel physiological functions of these ion-regulating proteins in the cardiovascular, nervous, and metabolic systems, their links to pathological conditions, and their mechanisms, aiming to apply these findings to drug therapies. The department is also well-equipped for experimental research (Figure 2). For more details on the professor's previous research, please visit

https://scholar.google.co.jp/citations?user=SYEk6r8AAAAJ&hl=ja

image01 image02

On the other hand, the Department of Pharmacology has been conducting research on pain control, pharmacological effects of analgesics, and pathophysiology of brain diseases for many years. Recently, studies have focused on molecular bases of somatosensory abnormalities of pain and mental/neurodegenerative diseases. Ion signaling plays a significant role in these areas, and there are interesting findings regarding the cardiotoxicity of neuroactive drugs. Future research will integrate these areas, using human iPSC-derived cardiomyocytes and neurons, to broadly explore the molecular basis of human cardiovascular and metabolic diseases and neurological disorders involving pain.

Dr. I-Shan Chen is conducting research on the structure-activity relationship of ion channels and receptors in neurons and cardiomyocytes and the regulatory mechanism of protein function by drugs by electrophysiological analysis using Xenopus oocytes as an in vitro expression system (Fig. 3).

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(Figure. 3)

Dr. Jumpei Yasuda is focused on organ fibrosis, where excessive extracellular matrix accumulates due to disease, and matricryptins derived from the breakdown of the extracellular matrix. His research primarily targets cardiovascular diseases such as heart failure and hypertension, exploring the pathogenesis of organ fibrosis and the functions of matricryptins, with the aim of developing new therapeutic drugs. This research includes analysis of heart function at the individual, tissue, and cellular levels (Figure 4).

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Dr. Takuya Notomi is advancing research on ion dynamics and mechanical stimuli in maintaining homeostasis and their impacts on diseases. Recently, he has been applying optogenetics to hard tissue research to investigate links with bone metabolic diseases. This approach can also be applied to cardiovascular disease research. He aims to develop drugs based on mechanisms that explore the relationship between various diseases and ion dynamics/mechanical stimuli, integrating molecular biology, morphology, and electrophysiological techniques (Figure 5).

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The department regularly conducts journal clubs. A designated person presents recent interesting publications, and through discussions among all staff, the latest information is leveraged to advance research.

Educational Overview

We provide fundamental knowledge on the mechanisms of various drugs in the body at the individual and cellular levels and the factors that influence drug efficacy. Our educational aim is to train medical professionals who can fully understand the pharmacological effects and adverse effects of drugs used in treatment, diagnosis, and prevention.

Courses in Charge School of Medicine l Living Organisms and Drugs, l Pharmacology Training, l Basic Medicine Problem-based Learning (PBL)
School of Health and Nursing Science l Basic Pharmacology, l Clinical Pharmacology
Graduate School of Medical and Pharmaceutical Sciences (including Graduate School of Medicine) l Introduction to Pharmacology, l Introduction to Medical Science Research Method

Staff (May 2024)

Professor Tomoe Nishitani (working name: Tomoe Y. Nakamura) tomoen@
Lecturer I-Shan Chen chenis@
Lecturer Takuya Notomi notomi@
Assistant Professor Jumpei Yasuda jpyasuda@
Research Assistant Mai Koike mkoike@
Ph.D. student Yuki Maeda m2388022@
Ph.D. student Mayu Suzuta m2488015@
Department Representative yakuri@

* E-mail domain (@wakayama-med.ac.jp)

Graduate Student Recruitment

The Department of Pharmacology is seeking graduate students (PhD and Master's programs). If you are interested in our research themes, please contact us. For detailed information on graduate application qualifications and recruitment, please visit the "Graduate School" section of our website.

Contact Information
Department of Pharmacology, Wakayama Medical University School of Medicine
Address: 811-1, Kimiidera, Wakayama, Wakayama 641-8509, Japan
Tel: 073-447-2300 (University representative, ext. 5256 for the department), 073-441-0629 (direct line)

Research Overview (2011~)

  • Research Themes
  • Papers (Original Articles and Review Articles)

2023年

  • Gando I, Becerra Flores M, Chen IS, Yang HQ, Nakamura TY, Cardozo TJ, Coetzee WA.
    CL-705G: a novel chemical Kir6.2-specific KATP channel opener Front Pharmacol, 20;14:1197257, 2023.
  • Mao D, Inoue H, Notomi T*, Goda S*.
    P38α contributes to TNF-α-induced IL-8 production in human gingival cells.Biofactors, 49(6):1223-1232, 2023.

2022年

  • Chen IS*, Eldstrom J, Fedida D, Kubo Y.
    A novel ion conducting route besides the central pore in an inherited mutant of G‐protein‐gated inwardly rectifying K+ channel.J Physiol, 600(3):603-622, 2022.

2021年

  • Notomi T*, Kobayashi R, Otsuka M, Kise C, Momota Y, Ezura Y, Kawazoe T.
    Light-induced Membrane Hyperpolarization Promotes Osteoblast Differentiation in MC3T3 Osteoblast-like Cells.
    J Hard Tissue Biol, 30: 4: 347-354, 2021
  • Notomi T*, Kise C, Kobayashi R, Otsuka M, Momota Y, Ezura Y, Kawazoe T.
    TPC1 and TPC2 Promote Osteoclastogenesis.
    J Hard Tissue Biol, 30: 4: 333-338, 2021
  • Notomi T*, Otsuka M, Kise C, Kobayashi R, Momota Y and Kawazoe T.
    Delivery of plasmid DNA into pre-osteoclast-like and mature osteoclast-like cells using cationic peptides.
    J Osaka Dent Univ, 55: 2: 239-243, 2021
  • 中村(西谷)友重*
    小児特有の心筋細胞内Ca2+シグナル調節機構とその治療標的としての可能性
    日本薬理学会誌, 2021: 156(6);346-350
  • 西谷(中村)友重*
    興奮性細胞における新規Ca2+シグナル制御因子NCS-1の多彩な生理機能と各種疾患との関連
    和歌山医学, 2021;72(4);192-197
  • Kiguchi N*, Fukazawa Y, Saika A, Uta D, Saika F, Nakamura TY, Ko MC, Kishioka S.
    Chemogenetic activation of central gastrin-releasing peptide-expressing neurons elicits itch-related scratching behavior in male and female mice.
    Pharmacology research & perspectives, 2021; 9; e00790
  • Saika F, Matsuzaki S, Kishioka S, Kiguchi N.
    Chemogenetic Activation of CX3CR1-Expressing Spinal Microglia Using Gq-DREADD Elicits Mechanical Allodynia in Male Mice Cells, 2021; 10; 874
  • Matsuki Y, Yanagawa T, Sumiyoshi H, Yasuda J, Nakao S, Goto M, Shibata-Seki T, Akaike T, Inagaki Y.
    Modification of exosomes with carbonate apatite and a glycan polymer improves transduction efficiency and target cell selectivity.
    Bioche Biophys Res Commun. 2021; 583 93-99
  • Sumiyoshi H, Okamura Y, Kawaguchi T A, Kubota T, Endo H, Yanagawa T, Yasuda J, Matsuki Y, Nakao S, Inagaki Y.
    External administration of moon jellyfish collagen solution accelerates physiological wound healing and improves delayed wound closure in diabetic model mice.
    Regenerative Therapy, 2021; 18 223-230

2020年

  • Kiguchi N*, Ding H, Ko MC.
    Therapeutic potentials of NOP and MOP receptor coactivation for the treatment of pain and opioid abuse.
    J Neurosci Res. 2020 Apr 7. [Epub ahead of print] Review. PMID: 32255240
  • Kiguchi N*,#, Uta D#, Ding H, Uchida H, Saika F, Matsuzaki S, Fukazawa Y, Abe M, Sakimura K, Ko MC, Kishioka S.
    Neuropharmacology. 2020 Mar 3;170:108025. PMID: 32142790
  • Kobayashi D*, Kiguchi N, Saika F, Kishioka S, Matsuzaki S*.
    Insufficient efferocytosis by M2-like macrophages as a possible mechanism of neuropathic pain induced by nerve injury.
    Biochem Biophys Res Commun. 2020 Feb 19. [Epub ahead of print] PMID: 32087968
  • 陳以珊*, 久保義弘.
    GIRKチャンネルの、小分子による新規機能調節機構.
    心電図 2020; 40(2);107-113.

2019年

  • Nakamura TY*, Nakao S, Wakabayashi S.
    Emerging Roles of neuronal Ca2+ Sensor-1 in Cardiac and neuronal Tissues:A Mini Review Front. Mol. Neurosci. 2019 12:56.
  • Kiguchi N, Ko MC*.
    Effects of NOP-Related Ligands in Nonhuman Primates.
    Handb Exp Pharmacol. 2019;254:323-343. PMID: 30879202
  • Kiguchi N#, Ding H#, Cami-Kobeci G, Sukhtankar DD, Czoty PW, DeLoid HB, Hsu FC, Toll L, Husbands SM*, Ko MC*.
    BU10038 as a safe opioid analgesic with fewer side-effects after systemic and intrathecal administration in primates.
    Br J Anaesth. 2019 Jun;122(6):e146-e156. PMID: 30916003
  • Saika F, Kiguchi N*, Matsuzaki S, Kobayashi D, Kishioka S.
    Inflammatory macrophages in the sciatic nerves facilitate neuropathic pain associated with type 2 diabetes mellitus.
    J Pharmacol Exp Ther. 2019 Mar;368(3):535-544. PMID: 30602591
  • Chen IS*, Liu C, Tateyama M, Karbat I, Uesugi M, Reuveny E, Kubo Y.
    Non-sedating antihistamines block G-protein-gated inwardly rectifying K+ channels.
    Br J Pharmacol. 2019; 176(17):3161-3179.
  • Furutani K, Tsumoto K, Chen IS, Handa K, Yamakawa Y, Sack J, Kurachi Y.
    Facilitation of IKr current by some hERG channel blockers suppresses early afterdepolarizations.
    J Gen Physiol. 2019; 151(2):214-230.
  • Ishii J, Suzuki A, Kimura T, Tateyama M, Tanaka T, Yazawa T, Arimasu Y, Chen IS, Aoyama K, Kubo Y, Saitoh S, Mizuno H, Kamma H.
    Congenital goitrous hypothyroidism is caused by dysfunction of the iodide transporter SLC26A7.
    Commun Biol. 2019; 2:270.
  • Yasuda J, Okada M, Yamawaki H.
    Protective effects of T3 peptide, an active fragment of tumstatin, against ischemia/reperfusion injury in rat heart.
    J. Pharmacol. Sci. 139: 193-200, 2019.
  • Notomi T*, Hiyama A, Nozaki T.
    Establishment of RANKL-highly sensitive RAW264 subclones in accordance with high expression of TPC2.
    J Transl Sci, 6, 2019. doi: 10.15761/JTS.1000357 (4 pages).
  • Notomi T*, Hiyama A, Nozaki T.
    Role of zinc and zinc-modulated ion channels, ORAI1 and HCN in osteoclasts.
    J Transl Sci, 6, 2019. doi: 10.15761/JTS.1000359 (4 pages)
  • Inubushi M, Notomi T*, Nozaki T. Retinoic acid promotes migration of MC3T3-E1 osteoblast-like cells via RARα signaling-mediated upregulation of profilin-1 expression.
    J Osaka Dent Univ, 53: 2: 149-156, 2019.
  • Notomi T*, Inubushi M, Nozaki T.
    Synaptotagmin VII RANKL-lysosomal vesicle fusion to the cell membrane in osteoblast-like cells.
    J Osaka Dent Univ, 53: 2: 141-147, 2019.

2018年

  • Kiguchi N*, Matsuzaki S, Saika F, Kobayashi D, Kishioka S.
    Epigenetic Regulation of Peripheral Macrophages in Neuropathic Pain.
    In: Epigenetics of Chronic Pain. Translational Epigenetics, 2018; 7: p49-68.
  • Saika F, Matsuzaki S, Kobayashi D, Kiguchi N*, Kishioka S.
    Chemokine CXCL1 is responsible for cocaine-induced reward in mice.
    Neuropsychopharmacol Rep. 2018 Sep;38(3):145-148. PMID: 30175527.
  • Ding H#, Kiguchi N#, Yasuda D, Daga PR, Polgar WE, Lu JJ, Czoty PW, Kishioka S, Zaveri NT*, Ko MC*.
    A bifunctional nociceptin and mu opioid receptor agonist is analgesic without opioid side effects in nonhuman primates.
    Sci Transl Med. 2018 Aug 29;10(456). pii: eaar3483. PMID: 30158150.
  • Ding H#, Kiguchi N*,#, Kishioka S, Ma T, Peters CM, Ko MC. Differential mRNAexpression of neuroinflammatory modulators in the spinal cord and thalamus oftype 2 diabetic monkeys.
    J Diabetes. 2018 Nov;10(11):886-895. PMID: 29749036.
  • Kiguchi N*, Kobayashi D, Saika F, Matsuzaki S, Kishioka S. Inhibition of peripheral macrophages by nicotinic acetylcholine receptor agonists suppresses spinal microglial activation and neuropathic pain in mice with peripheral nerve injury.
    J Neuroinflammation. 2018 Mar 27;15(1):96. PMID: 29587798.
  • Saika F, Kiguchi N*, Wakida N, Kobayashi D, Fukazawa Y, Matsuzaki S, Kishioka S.
    Upregulation of CCL7 and CCL2 in reward system mediated through dopamine D1 receptor signaling underlies methamphetamine-induced place preference in mice.
    Neurosci Lett. 2018 Feb 5;665:33-37. PMID: 29174638.
  • Chen IS* and Kubo Y.
    Ivermectin and its target molecules: shared and unique modulation mechanisms of ion channels and receptors by ivermectin.
    J Physiol. 2018; 596(10):1833-1845.

2017年

  • Nakamura TY*, Nakao S, Nakajo Y, Takahashi JC, Wakabayashi S, Yanamoto H:
    Possible Signaling Pathways Mediating Neuronal Calcium Sensor-1-Dependent Spatial Learning and Memory in Mice. PLoS ONE, 2017 12(1) e0170829.
  • Kiguchi N*, Kobayashi D, Saika F, Matsuzaki S, Kishioka S.
    Pharmacological Regulation of Neuropathic Pain Driven by Inflammatory Macrophages.
    Int J Mol Sci. 2017 Nov 1;18(11). pii: E2296. Review. PMID: 29104252.
  • Kiguchi N*, Sakaguchi H, Kadowaki Y, Saika F, Fukazawa Y, Matsuzaki S, Kishioka S.
    Peripheral administration of interleukin-13 reverses inflammatory macrophage and tactile allodynia in mice with partial sciatic nerve ligation.
    J Pharmacol Sci. 2017 Jan;133(1):53-56. PMID: 28057412.
  • Kiguchi N, Ding H, Peters CM, Kock ND, Kishioka S, Cline JM, Wagner JD, Ko MC*.
    Altered expression of glial markers, chemokines, and opioid receptors in the spinal cord of type 2 diabetic monkeys.
    Biochim Biophys Acta. 2017 Jan;1863(1):274-283. PMID: 27751964.
  • Chen IS*, Tateyama M, Fukata Y, Uesugi M, Kubo Y.
    Ivermectin activates GIRK channels in a PIP2-dependent, Gβγ-independent manner and an amino acid residue at the slide helix governs the activation.
    J Physiol. 2017; 595(17):5895-5912.
  • Chen IS, Furutani K, Kurachi Y.
    Structural determinants at the M2 muscarinic receptor modulate the RGS4-GIRK response to pilocarpine by impairment of the receptor voltage sensitivity.
    Sci Rep. 2017; 7(1):6110.
  • Tsukamoto H, Chen IS, Kubo Y, Furutani Y.
    A ciliary opsin in the brain of a marine annelid zooplankton is ultraviolet-sensitive, and the sensitivity is tuned by a single amino acid residue.
    J Bio Chem. 2017; 292(31):12971-12980.
  • Yasuda J, Fukui K, Okada M, Yamawaki H.
    T3 peptide, a fragment of tumstatin, stimulates proliferation and migration of cardiac fibroblasts through activation of Akt signaling pathway.
    Naunyn-Schmiedeberg's Arch. Pharmacol. 390: 1135-1144, 2017.
  • Yasuda J, Okada M, Yamawaki H.
    T3 peptide, an active fragment of tumstatin, inhibits H2O2-induced apoptosis in H9c2 cardiomyoblasts.
    Eur. J. Pharmacol. 807: 64-70, 2017.
  • Okada M, Imoto K, Sugiyama A, Yasuda J, Yamawaki H.
    New Insights into the Role of Basement Membrane-Derived Matricryptins in the Heart.Biol. Pharm. Bull. 40: 2050-2060, 2017.
  • Notomi T*, Kuno M, Hiyama A, Nozaki T, Ohura K, Ezura Y, Noda M.
    Role of lysosomal channel protein TPC2 in osteoclast differentiation and bone remodeling under normal and low-magnesium conditions.
    J Biol Chem, 292: 12: 20998-21010, 2017.

2016年

  • Nakamura TY*, Nakao S, Wakabayashi S.
    Neuronal Ca2+ sensor-1 contributes to stress tolerance in cardiomyocytes via activation of mitochondrial detoxification pathways.
    J. Mol Cell Cardiol. 2016 99:23-34.
  • Ding H, Czoty PW, Kiguchi N, Cami-Kobeci G, Sukhtankar DD, Nader MA, Husbands SM, Ko MC*.
    A novel orvinol analog, BU08028, as a safe opioid analgesic without abuse liability in primates.
    Proc Natl Acad Sci U S A. 2016 Sep 13;113(37):E5511-8. PMID: 27573832.
  • Kiguchi N, Ding H, Ko MC*.
    Central N/OFQ-NOP Receptor System in Pain Modulation.
    Adv Pharmacol. 2016;75:217-43. Review. PMID: 26920014.
  • Kiguchi N, Sukhtankar DD, Ding H, Tanaka K, Kishioka S, Peters CM, Ko MC*.
    Spinal Functions of B-Type Natriuretic Peptide, Gastrin-Releasing Peptide, and Their Cognate Receptors for Regulating Itch in Mice.
    J Pharmacol Exp Ther. 2016 Mar;356(3):596-603. PMID: 26669425.
  • Nakamoto T, Izu Y, Kawasaki M, Notomi T, Hayata T, Noda M, Ezura Y.
    Mice deficient in CIZ/NMP4 develop an attenuated from of K/BxN-serum induced arthritis.
    J Cell Biochem, 117: 4: 970-7, 2016.

2015年

  • Kobayashi S, Nakamura TY, Wakabayashi S*.
    Calcineurin B homologous protein 3 negatively regulates cardiomyocyte hypertrophy via inhibition of glycogen synthase kinase 3 phosphorylation.
    J. Mol. Cell. Cardiol., 2015, 84:133-42.
  • Nakao S, Wakabayashi S, Nakamura, TY*.
    Stimulus-dependent regulation of nuclear Ca2+ signaling in cardiomyocytes: a role of neuronal calcium sensor-1.
    PLoS ONE, 2015 10(4):e0125050.
  • Kiguchi N, Saika F, Kobayashi Y, Ko MC, Kishioka S*.
    TC-2559, an α4β2 nicotinic acetylcholine receptor agonist, suppresses the expression of CCL3 and IL-1β through STAT3 inhibition in cultured murine macrophages.
    J Pharmacol Sci. 2015 Jun;128(2):83-6. PMID: 26012743.
  • Kobayashi Y, Kiguchi N, Fukazawa Y, Saika F, Maeda T, Kishioka S*.
    Macrophage-T cell interactions mediate neuropathic pain through the glucocorticoid-induced tumor necrosis factor ligand system.
    J Biol Chem. 2015 May 15;290(20):12603-13. PMID: 25787078.
  • Kiguchi N, Kobayashi Y, Saika F, Sakaguchi H, Maeda T, Kishioka S*.
    Peripheral interleukin-4 ameliorates inflammatory macrophage-dependent neuropathic pain.
    Pain. 2015 Apr;156(4):684-93. PMID: 25630024.
  • Saika F, Kiguchi N, Kobayashi Y, Kishioka S*.
    Peripheral alpha4beta2 nicotinic acetylcholine receptor signalling attenuates tactile allodynia and thermal hyperalgesia after nerve injury in mice.
    Acta Physiol (Oxf). 2015 Feb;213(2):462-71. PMID: 25491757.
  • Yasuda J, Okada M, Yamawaki H.
    Endostatin inhibits T-type Ca2+ channel current in guinea pig ventricular myocyte.
    J. Vet. Med. Sci. 77: 1289-1291, 2015.
  • Yasuda J, Takada L, Kajiwara Y, Okada M, Yamawaki H.
    Endostatin inhibits bradykinin-induced cardiac contraction.
    J. Vet. Med. Sci. 77: 1391-1395, 2015.
  • Notomi T*, Kuno M, Hiyama A, Ezura Y, Honma M, Ishizuka T, Ohura K, Yawo H, Noda M.
    Membrane depolarization regulates intracellular RANKL transport in non-excitable osteoblasts.
    Bone, 81: 1: 306-14, 2015.
  • Notomi T*, Kuno M, Hiyama A, Ohura K, Noda M, Skerry TM.
    Zinc-induced effects on osteoclastogenesis involves activation of hyperpolarization-activated cyclic nucleotide modulated channels via changes in membrane potential.
    J Bone Miner Res, 30: 9: 1618-26, 2015.
  • Aryal S, Miyai K, Izu Y, Hayata T, Notomi T, Noda M, Ezura Y.
    Nck influences preosteoblastic/osteoblastic migration and bone mass.
    Proc Natl Acad Sci USA, 112: 50: 15432-7, 2015.
  • Kawasaki M, Ezura Y, Hayata T, Notomi T, Izu Y, Noda M.
    TGF-Beta Suppresses Ift88 Expression in Chondrocytic ATDC5 cells.
    J Cell Physiol, 230: 11: 2788-95, 2015.
  • Yamada T, Ezura Y, Hayata T, Moriya S, Shirakawa J, Notomi T, Aryal S, Kawasaki M, Izu Y, Harada K, Noda M.
    β2 Adrenergic receptor activation suppresses BMP-induced alkaline phosphatase expression in osteoblast-like MC3T3E1 cells.
    J Cell Biochem, 116: 6: 1144-52, 2015.
  • Moriya S, Hayata T, Notomi T, Aryal S, Nakamoto T, Izu Y, Kawasaki M, Yamada T, Shirakawa J, Kaneko K, Ezura Y, Noda M.
    PTH regulates β2-adrenergic receptor expression in osteoblast-like MC3T3-E1 cells.
    J Cell Biochem, 116: 1: 142-8, 2015.
  • 雑賀史浩, 木口倫一, 岸岡史郎.
    The role of CC-chemokine ligand 2 in the development of psychic dependence on methamphetamine.
    日本アルコール薬物医学会雑誌 2015; 50(5): 189-195.
  • 小林悠佳, 木口倫一, 雑賀史浩, 岸岡史郎.
    Exploration of novel therapeutic targets for neuropathic pain based on the regulation of immune cells.
    日本神経精神薬理学雑誌 2015; 35(3): 65-72.
  • 小林悠佳, 岸岡史郎.
    新規疼痛制御分子としてのマクロファージ由来マトリックスメタロプロテアーゼ12 の可能性.
    PAIN RESEARCH. 2015; 30(1): 37-45.

2014年

  • Kuramoto K, Sakai F, Yoshinori N, Nakamura TY, Wakabayashi S, Kojidani T, Haraguchi T, Hirose F, Osumi T*.
    Deficiency of a lipid droplet protein, perilipin 5, suppresses myocardial lipid accumulation, thereby preventing type 1 diabetes-induced heart malfunction.
    Mol. Cell. Biol., 2014 34:2721-31.
  • Shimada-Shimizu N, Hisamitsu T, Nakamura TY, Hirayama N, Wakabayashi S*.
    Na+/H+ exchanger 1 is regulated via its lipid-interacting domain, which functions as a molecular switch: a pharmacological approach using indolocarbazole compounds.
    Mol. Pharmacol., 2014, 85:18-28.
  • Wakida N, Kiguchi N*, Saika F, Nishiue H, Kobayashi Y, Kishioka S.
    CC-chemokine ligand 2 facilitates conditioned place preference to methamphetamine through the activation of dopamine systems.
    J Pharmacol Sci. 2014;125(1):68-73. PMID: 24748435.
  • Ueno K, Kiguchi N, Kobayashi Y, Saika F, Wakida N, Yamamoto C, Maeda T, Ozaki M, Kishioka S*.
    Possible involvement of endogenous opioid system located downstream of α7 nicotinic acetylcholine receptor in mice with physical dependence on nicotine.
    J Pharmacol Sci. 2014;124(1):47-53. PMID: 24366190.
  • Kiguchi N, Kobayashi Y, Kadowaki Y, Fukazawa Y, Saika F, Kishioka S*.
    Vascular endothelial growth factor signaling in injured nerves underlies peripheral sensitization in neuropathic pain.
    J Neurochem. 2014 Apr;129(1):169-78. PMID: 24304382.
  • Kiguchi N, Saika F, Kobayashi Y, Kishioka S*.
    Epigenetic regulation of CC-chemokine ligand 2 in nonresolving inflammation.
    Biomol Concepts. 2014 Aug;5(4):265-73. Review. PMID: 25372758.
  • Kishioka S*, Kiguchi N, Kobayashi Y, Saika F.
    Nicotine effects and the endogenous opioid system.
    J Pharmacol Sci. 2014;125(2):117-24. Review. PMID: 24882143.
  • Chen IS, Furutani K, Inanobe A, Kurachi Y.
    RGS4 regulates partial agonism of the M2 muscarinic receptor-activated K+ currents.
    J Physiol. 2014; 592(6): 1237-1248.
  • Notomi T*, Karasaki I, Okazaki Y, Okimoto N, Kato Y, Ohura K, Noda M, Nakamura T, Suzuki M.
    Insulinogenic sucrose + amino acid mixture ingestion immediately after resistance exercise has an anabolic effect on bone compared with non-insulinogenic fructose + amino acid mixture in growing rats.
    Bone, 65: 1: 42-48, 2014.
  • Shirakawa J, Ezura Y, Moriya S, Kawasaki M, Yamada T, Notomi T, Nakamoto T, Hayata T, Miyawaki A, Omura K, Noda M.
    Migration linked to FUCCI-indicated cell cycle is controlled by PTH and mechanical stress.
    J Cell Physiol, 229: 10: 1353-8, 2014.
  • Watanabe C, Morita M, Ezura Y, Nakamoto T, Hayata T, Kikuguchi C, Xue L, Kobayashi Y, Takahashi N, Notomi T, Moriyama K, Tadashi Yamamoto, Noda M.
    Stability of mRNA influences osteoporotic bone mass via Cnot3.
    Proc Natl Acad Sci USA, 111: 7: 2692-7, 2014.
  • 西谷(中村)友重*、若林繁夫
    幼少期の心機能を制御する新しいCa2+調節タンパク質の発見とその分子機構
    日本小児循環器学会雑誌, 2014, 30(3):224-231.
  • 木口倫一, 岸岡史郎.
    傷害末梢神経におけるヒストン修飾は神経障害性疼痛を調節する.
    PAIN RESEARCH. 2014; 29(1): 9-16.
  • 岸岡史郎, 木口倫一, 小林悠佳, 雑賀史浩, 山本千鶴子.
    The development of physical dependence on nicotine and endogenous opioid system--participation of alpha7 nicotinic acetylcholine receptor.
    日本アルコール薬物医学会雑誌 2014; 49(5): 227-237.
  • 納富拓也(宮村実晴編)、ニュー運動生理学(運動と骨格:骨形態)、真興交易(株)医書出版部、 262-270頁、2014

2013年

  • Shimada-Shimizu N, Hisamitsu T, Nakamura TY, Wakabayashi S*.
    Evidence that Na+ /H+ exchanger 1 is an ATP-binding protein.
    FEBS J. 2013, 280:1430-42.
  • Kiguchi N, Kobayashi Y, Saika F, Kishioka S*.
    Epigenetic upregulation of CCL2 and CCL3 via histone modifications in infiltrating macrophages after peripheral nerve injury.
    Cytokine. 2013 Dec;64(3):666-72. PMID: 24135048.
  • Kishioka S*, Kiguchi N, Kobayashi Y, Yamamoto C, Saika F, Wakida N, Ko MC, Woods JH.
    Pharmacokinetic evidence for the long-lasting effect of nor-binaltorphimine, a potent kappa opioid receptor antagonist, in mice.
    Neurosci Lett. 2013 Sep 27;552:98-102. PMID: 23933210.
  • Maeda T*, Ozaki M, Kobayashi Y, Kiguchi N, Kishioka S.
    HMGB1 as a potential therapeutic target for neuropathic pain.
    J Pharmacol Sci. 2013;123(4):301-5. Review. PMID: 24285082.
  • Wakabayashi S*, Hisamitsu T, Nakamura TY.
    Regulation of the cardiac Na+/H+ exchanger in health and disease.
    J. Mol. Cell. Cardiol. 2013, 61:68-76
  • Suzuki T, Notomi T*, Miyajima D, Mizoguchi F, Hayata T, Nakamoto T, Hanyu R, Kamolratanakul P, Mizuno A, Makoto Suzuki, Ezura Y, Izumi Y, Noda M.
    Osteoblastic differentiation enhances expression of TRPV4 that is required for calcium oscillation induced by mechanical force.
    Bone, 54: 1: 172-8, 2013.
  • Aryal S, Miyai K, Ezura Y, Hayata T, Notomi T, Nakamoto T, Pawson T, Noda M.
    Nck1 deficiency accelerates unloading-induced bone loss.
    J Cell Physiol, 228: 7: 1397-1403, 2013.

2012年

  • Kuramoto K, Okamura T, Yamaguchi T, Nakamura TY, Wakabayashi S, Morinaga H,
    Nomura M, Yanase T, Otsu K, Usuda N, Matsumura S, Inoue K, Fushiki T, Kojima Y,
    Hashimoto T, Sakai F, Hirose F, Osumi T*.
    Perilipin 5, a lipid droplet-binding protein, protects the heart from oxidative burden by sequestering fatty acid from excessive oxidation.
    J. Biol. Chem. 2012, 287:23852-63.
  • Hisamitsu T, Nakamura TY, Wakabayashi S*.
    Na+/H+ exchanger 1 directly binds to calcineurin a and activates downstream NFAT signaling, leading to cardiomyocyte hypertrophy.
    Mol. Cell. Biol. 2012, 32:3265-80.
  • Kiguchi N, Kobayashi Y, Maeda T, Tominaga S, Nakamura J, Fukazawa Y, Ozaki M, Kishioka S*.
    Activation of nicotinic acetylcholine receptors on bone marrow-derived cells relieves neuropathic pain accompanied by peripheral neuroinflammation.
    Neurochem Int. 2012 Dec;61(7):1212-9. PMID: 22989685.
  • Saika F, Kiguchi N*, Kobayashi Y, Fukazawa Y, Kishioka S.
    CC-chemokine ligand 4/macrophage inflammatory protein-1β participates in the induction of neuropathic pain after peripheral nerve injury.
    Eur J Pain. 2012 Oct;16(9):1271-80. PMID: 22528550.
  • Kobayashi Y, Kiguchi N, Maeda T, Ozaki M, Kishioka S*. The critical role of spinal ceramide in the development of partial sciatic nerve ligation-induced neuropathic pain in mice.
    Biochem Biophys Res Commun. 2012 May 4;421(2):318-22. PMID: 22503971.
  • Ueno K, Maeda T, Kiguchi N, Kobayashi Y, Ozaki M, Kishioka S*.
    Availability of serum corticosterone level for quantitative evaluation of morphine withdrawal in mice.
    Drug Discov Ther. 2011 Apr;5(2):71-5. PMID: 22466143.
  • Iwai S, Kiguchi N*, Kobayashi Y, Fukazawa Y, Saika F, Ueno K, Yamamoto C, Kishioka S.
    Inhibition of morphine tolerance is mediated by painful stimuli via central mechanisms.
    Drug Discov Ther. 2012 Feb;6(1):31-7. PMID: 22460426.
  • Kiguchi N, Kobayashi Y, Maeda T, Fukazawa Y, Tohya K, Kimura M, Kishioka S*.
    Epigenetic augmentation of the macrophage inflammatory protein 2/C-X-C chemokine receptor type 2 axis through histone H3 acetylation in injured peripheral nerves elicits neuropathic pain.
    J Pharmacol Exp Ther. 2012 Mar;340(3):577-87. PMID: 22135382.
  • Kiguchi N, Kobayashi Y, Kishioka S*.
    Chemokines and cytokines in neuroinflammation leading to neuropathic pain.
    Curr Opin Pharmacol. 2012 Feb;12(1):55-61. Review. PMID: 22019566.
  • Nakamura TY*, Wakabayashi S.
    Role of neuronal calcium sensor-1 in the cardiovascular system.
    Trends Cardiovasc. Med 2012., 22:12-17.
  • Nakamura TY*, Wakabayashi S. Diverse functions of neuronal calcium sensor-1 (NCS-1) in excitable cells. Trends in Cell & Molecular Biology 2012, 7:99-110.
  • Notomi T*, Ezura Y, Noda M.
    Identification of two pore channel 2 as a novel regulator of osteoclastogenesis.
    J Biol Chem, 287: 42: 35057-64, 2012.
  • Miyajima D, Hayata T, Suzuki T, Hemmi H, Ezura Y, Nakamoto T, Notomi T, Amagasa T, B?ttcher RT, Costell M, F?ssler R, Noda M.
    Profilin1 regulates sternum development and endochondral bone formation.
    J Biol Chem, 287: 40: 33545-53, 2012.
  • Hanyu R, Wehbi VL, Hayata T, Moriya S, Feinsteind TN, Ezura Y, Nagao M, Saita Y, Hemmi H, Notomi T, Nakamoto T, Schipani E, Takeda S, Kaneko K, Kurosawa H, Karsenty G, Kronenberg HM, Vilardaga JP, Noda M.
    Anabolic action of parathyroid hormone regulated by the beta2-adrenergic receptor.
    Proc Natl Acad Sci USA,109: 19: 7433-8, 2012.
  • Sakuma T, Nakamoto T, Hemmi H, Kitazawa S, Kitazawa R, Notomi T, Hayata T, Ezura Y, Amagasa T, Noda M.
    CIZ/NMP4 is expressed in B16 melanoma and forms a positive feedback loop with RANKL to promote migration of the melanoma cells.
    J Cell Physiol, 227: 7: 2807-12, 2012.
  • Yumoto K, Nifuji A, Rittling S, Tsuchiya Y, Kon S, Uede T, Denhardt DT, Hemmi H, Notomi T, Hayata T, Ezura Y, Nakamoto T, Noda M.
    Osteopontin deficiency suppresses tumor necrosis factor-α–induced apoptosis in chondrocytes.
    Cartilage, 3: 1: 79-85, 2012.
  • 木口倫一.
    炎症性ケモカインによる慢性神経炎症を介した神経障害性疼痛形成の分子基盤
    和歌山医学. 2012; 63(3): 98-103. 和歌山医学会.

2011年

  • Nakamura TY* , Jeromin A, Mikoshiba K, Wakabayashi S.
    Neuronal calcium sensor-1 promotes immature heart function and hypertrophy by enhancing Ca2+ signals.
    Circ. Res. 2011, 109:512-23.
  • Yamamoto A, Kiguchi N*, Kobayashi Y, Maeda T, Ueno K, Yamamoto C, Kishioka S.
    Pharmacological relationship between nicotinic and opioid systems in analgesia and corticosterone elevation.
    Life Sci. 2011 Dec 19;89(25-26):956-61. PMID: 22036617.
  • Chen IS, Dai ZK, Welsh DG, Chen IJ, Wu BN.
    Protein kinases modulate store- operated channels in pulmonary artery smooth muscle cells.
    J Biomed Sci. 2011; 18(2):1-12.
  • Nagao M, Feinstein TN, Ezura Y, Hayata T, Notomi T, Saita Y, Hanyu R, Hemmi H, Izu Y, Takeda S, Wang K, Rittling S, Nakamoto T, Kaneko K, Kurosawa H, Karsenty G, Denhardt DT, Vilardaga JP, Noda M.
    Sympathetic control of bone mass regulated by osteopontin.
    Proc Natl Acad Sci USA, 108: 43: 17767-72, 2011.
  • Kondo H, Ezura Y, Nakamoto T, Hayata T, Notomi T, Sorimachi H, Takeda S, Noda M.
    MURF1 deficiency suppresses unloading-induced effects on osteoblasts and osteoclasts to lead to bone loss.
    J Cell Biochem, 112: 12: 3525-30, 2011.
  • Hanyu R, Hayata T, Nagao M, Saita Y, Hemmi H, Notomi T, Nakamoto T, Schipani E, Kronenberg H, Kaneko K, Kurosawa H, Ezura Y, Noda M.
    Per-1 is a specific clock gene regulated by parathyroid hormone (PTH) signaling in osteoblasts and is functional for the transcriptional events induced by PTH.
    J Cell Biochem, 112: 2: 433-8, 2011.
  • Kamolratanakul P, Hayata T, Ezura Y, Kawamata A, Hayashi C, Yamamoto Y, Hemmi H, Nagao M, Hanyu R, Notomi T, Nakamoto T, Amagasa T, Akiyoshi K, Noda M.
    Nanogel-based scaffold delivery of prostaglandin E(2) receptor-specific agonist in combination with a low dose of growth factor heals critical-size bone defects in mice.
    Arthritis Rheum, 63: 4: 1021-33, 2011.
  • Morishita M, Ono N, Miyai K, Nakagawa T, Hanyu R, Nagao M, Kamolratanakul P, Notomi T, Rittling S, Denhardt DT, Kronenberg HM, Ezura Y, Hayata T, Nakamoto T, Noda M.
    Osteopontin deficiency enhances parathyroid hormone/parathyroid hormone related peptide receptor (PPR) signaling-induced alteration in tooth formation and odontoblastic morphology.
    Tissue Cell, 43: 3: 196-200, 2011.
  • Nagao M, Saita Y, Hanyu R, Hemmi H, Notomi T, Hayata T, Nakamoto T, Nakashima K, Kaneko K, Kurosawa H, Ishii S, Ezura Y, Noda M.
    Schnurri-2 deficiency counteracts against bone loss induced by ovariectomy.
    J Cell Physiol, 226: 3: 573-8, 2011.
  • 木口倫一, 岸岡史郎.
    神経炎症が関与する末梢神経障害性疼痛のメカニズム.
    ペインクリニック. 2011; 32 (10): pp1499-1509. 真興交易(株).

Research Overview (2011~)

Laboratory Staff

FY2024 Students in Basic Assignment (3rd year medical students)

photo01

Ms. Maeda, a graduate student, received the Best Oral Presentation Award at the 10th Annual Meeting of the Physiological Society of Asia and Oceania (FAOPS2023).

April, 2024: Cherry-blossom viewing